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Name:   Douaa Mohammed Sayed

Position: Professor and head of Clinical Pathology Department

                   Vice Dean for community service and environmental development – Faculty of Medicine  

                Suez University

Date of Birth: 23/11/1971

Marital Status: Married

e-mails: douaa.sayed@med.suezuni.edu.eg ; douaa_sayed@hotmail.com ; douaa.mohammed@57357.org

She has Master and M.D. in Clinical Pathology from Faculty of Medicine and South Egypt Cancer Institute Assiut University, Egypt.

She established and developed the first flow cytometry lab accredited by ISO15189 in the middle East. She also established and was the Executive manager for the first Bone Marrow Transplantation centre and biorepresitory units in South Egypt.

She obtained more than 3 funded research projects as a PI in the field of Immunology and Cancer Biology and also 3 projects in the field of Laboratory accreditation. 

She awarded the Incentive (Encouraging) National Award in Medicine Science 2011 and also the best research in Medical laboratory and Hematology 2013, from Academy of Scientific Research and Technology, Egypt.

She established and was the associated editor for SECIOncology journal, editor (Journal of hematological malignancies) and reviewer in more than 6 other peer-reviewed international journals.

She supervised more than 30 M.D and 17 master theses in the field of flow cytometry. For the publication record, she published more than 90 papers in peer-reviewed high impact international journals within the field of interest.

- M.B.B.Ch: Assiut University; 1995.                        

- Msc. of Clinical Pathology: Faculty of Medicine, Assiut University; 2000.

- M.D. in Clinical Pathology and hematological malignancy: South Egypt Cancer Institute-Assiut University; 2005

- MBA (Hospital Management): Arab Academy for Science, Technology and Maritime Transport.

- Professional Diploma in Hospital Management: Missouri State University.

September 2009: award for the best research in Oncology field, Assiut University

Award for international publication of the year 2009 from SECI, Assiut University

Award for international publication of the year 2010 from SECI, Assiut University

State Incentive Award for Medical Sciences 2011.

Award for best research in Medical laboratory and hematology 2013.

1. The 4^th – 7^th international flow cytometry workshops, BMT Lab, National Cancer Institute, Cairo.
2. Oral presentations in The 1^st- 20^th international conference of the Egyptian society of hematology & research
3. Training on COM.TEC – AS.TEC (Fresenius HemoCare) in Dreieich / Germany, 2001.
4. Trainer in the first (Basic and principals of flow cytometry), 2nd (Clinical Applications of Flow Cytometry) 2004.
5. Annual conference of the Egyptian Society of Hematology and Research.
6. Annual conference of Clinical Pathology Department, Faculty of Medicine, Assiut University
7. Annual conference of the Egyptian Society of Laboratory Medicine
8. Annual conference of the Egyptian Society of Hematology
9. First international transfusion medicine congress, Cairo, March, 2006
10. Trainer in the 2nd (Clinical Applications of Flow Cytometry) 27-28 March 2006 and third (Flow cytometry: a sensitive analytic tool for small populations) 12-14 Feb. 2008. Flow cytometry workshops in South Egypt Cancer Institute, Assiut University.
11. Second Workshop Health Collaborative Program (Immunology & Epidemiology workshop) WHO/EMRO, Cairo, 2006
12. Training on statistical methods in medical sciences, Assiut, 2006
13. Trainer in third (Flow cytometry: a sensitive analytic tool for small populations) 12-14 Feb. 2008. Flow cytometry workshops in South Egypt Cancer Institute, Assiut University.
14. Proposal writing for international funding workshop, British council, March 2008
15. Oral presentation of an abstract titled (Effect of major burn on immunophenotype of peripheral blood T-lymphocytes) at ISBI 2008, Montreal, Canada.
16. Workshop of " Performance driven quality control" 28-30 March, 2009- Cairo, Egypt
17. Oral presentation of an abstract titled (Flow cytometric platelet cross-matching to predict successful platelet transfusion in acute leukemia) and for hemato-morphology cases and poster presentation of an abstract titled (Monocyte-platelet aggregates and platelet micro-particles in patients with post-hepatic liver cirrhosis) at ISH 2009, Cairo, Egypt.
18. Workshop of "Laboratory Bio-safety and its level" in Molecular biology research unit with collaboration with Virginia Tech University, 27-28/12/2009 Assiut.
19. First Annual Scientific conference of the Clinical & Chemical Pathology Department Cairo University at 31 March-2 April, 2010 (Accredited by UEMS-EACCME event#3882) Credit hours earned 4:20.
20. 56^th Annual SSC meeting (International Society on thrombosis and haemostasis) 22-25 May 2010 Cairo. 21 European CME credits (ECMEC).
21. Training program of “Student Evaluation” 20-22 July 2010 Assiut University.
22. Workshop of “Bone Marrow Pathology: Difficult to diagnose bone marrow lesions” 10-12 October 2010 Cairo University
23. Workshop of “Recent advances of Pathology Services in Cancer Management” 21 October 2010 SECI Assiut University (CME 10 credit points).
24. Second international conference, Clinical Oncology Department Assiut University 1-4 December 2010.
25. First Quality Seminar “Creating a culture of quality in the medical laboratory” BMT lab, NCI Cairo University 8-December 2010.
26. Training program of “e-learning” 28-30 December 2010 Assiut University.
27. Flow Cytometry workshop at King Fahad Specialist Hospital- Dammam, Saudi Arabia 16-17 April 2011 Accredited by Saudi Commission for Health Specialties for 11 CME hours (Accreditation No. 17555/2011) Dated 6 March 2011).
28. Training program of “Strategic planning” 11-13 October 2011 Assiut University
29. Workshop of “ISO 15189 in medical laboratories” 29 October 2011 SECI Assiut University.
30. Training course: “ISO 15189:2007 Standard” 16^th - 19^th Nov 2011, The Egyptian society for quality.
31. Workshop of “Difficulties and Risk Management of Quality in Medical Lab” 15-16 Feb 2012 SECI Assiut University.
32. Training course “ISO 15189:2007 Internal Audit”. 21-25^th Feb 2012. The Egyptian society for quality.
33. Training course “Safety in Medical Lab” March 2012.
34.  1^st Middle East EUROFLOW INFINICYTE WORKSHOP APRIL 2nd-3rd 2012 DUBAI- U.A.E
35. 4th Congress of the European Academy of Paediatric Societies in Istanbul, Turkey from October 5-9, 2012
36. A workshop on “proficiency testing as a necessary requirement for labs accreditation” 20^th Oct. 2012 Assiut University.
37.  A workshop on ISO 15189 in Blood Banks. 4-5^th Nov. 2012
38. Educational session “Is your laboratory’s quality on the mark? CAP inspection preparation seminar. 26^th Jan, 2013 in American Hospital, Dubai, United Arab Emirates
39.  2^nd Middle East EUROFLOW INFINICYTE WORKSHOP May 4-5^th 2013 DUBAI- U.A.E
40. Training course “Understanding and implementing ISO 15189:2012” 14-18^th August 2013.
41. Trainer in “New practical application of 8 color flow cytometry” at 11-12^th Feb 2014, Assiut.
42. Training course “Internal Audit for ISO 15189:2012” 23^th - 25^th March 2014.
43. Training on FACSCanto Diva 7. 16-19^th June 2014 at Heidelberg, Germany.
44.  Middle East Annual TERUMOBCT customers meeting, Athens 22-26^th Sept 2014.
45.  Trainer in “Detection of Apoptosis and cell cycle by flow cytometry” workshop. 20^th Nov. 2014 at MERC Assiut University.
46. Trainer in "Applications of Flow cytometry in research & diagnosis" workshop. 15^th – 17^th August, 2015 at Center of Excellence in cancer research, Tanta University, Egypt.
47. Speaker at the 39^th conference of the Egyptian Society of histology &cytology "Flow cytometry in stem cell transplantation" December 17-18, 2015, Assiut.
48. Speaker at the 5^th pan Arab hematology association congress ' Telomere length assessed by flow FISH in patients with acute lymphoblastic leukemia: Indicative of disease progression and overall survival" and "Flow cytometry Egyptian Report" 11^th – 13^th Feb, 2016, Cairo, Egypt.
49. President of Bone marrow transplantation workshop, 17^th – 20^th April, 2016.
50. General Secretory of 1^st Pan Arab Flow Cytometry Working Group workshop held on Oct 14-15, 2016, Grand Nile Tower, Cairo.
51. President of workshop on Updates in Hematopathology, 11-12^th Jan, 2017, SECI, Assiut, Egypt.
52. 6^th Pan Arab Hematology congress. 11-13^th Feb, 2017, Kuwait.
53. Trainer at Immunophenotyping and Flowcytometry workshop, 12^th Feb, 2017, Kuwait.
54. Chairperson at EHOG session, 8^th Annual International Conference of Clinical Oncology Department, Assiut University. 21-24^th Feb. 2017.
55. Chairperson at 7^th Annual conference of Clinical Pathology Department, Alexandria University, 7-8^th March 2017.  Alexandria, Egypt.
56. Speaker at the 13^th Scientific conference of Clinical Pathology Department, Suez Canal University "Accreditation of Laboratories" 26^th March, 2017, Ismailia, Egypt.
57. Speaker at 28^th Annual conference of ESLM, "Tricky normal and abnormal cell population in MDS immunophenotyping' 8-10^th April 2017, Cairo, Egypt.
58. Speaker and chairperson at 2^nd annual conference of Faculty of Medicine – Alazhar University Assiut. 12^th – 13^th April 2017, Assiut.
59. Trainer in "Bone marrow transplantation workshop" 17^th – 20^th April, 2016 at South Egypt Cancer Institute, Assiut University.
60. Workshop course "International University Rankings" at the Military Medical Academy on 6^th & 7^th Jan, 2017.
61. Training in Code of Ethics held on 11-12/April/2017. SECI, Assiut University
62. Training in Flow cytometry quality management system held on 9-11/May/2017. SECI, Assiut University.
63. Speaker at Advances in Stem Cell Researches Conference held on 18^th – 19^th May 2017.
64. Training in Health& Safty & Fire fighting held on 16-17/July/2017. SECI, Assiut University
65. Assessor training course for” Accreditation of Medical Laboratories According to ISO 15189:2012 & EGAC Requirements” from 6 to 9 August, 2017, EGAC, Cairo.
66. Conference secretory of 8-10^th International scientific conference of SECI. 4-5^th Oct. 2017.
67. Trainer at Biorepresitory workshop held on 4^th Oct. 2017, Assiut University, Assiut, Egypt.
68. Speaker and chairperson at 10^th BGICC (research course) "Developing biobank in developing countries" 18-19^th Jan 2018, Cairo, Egypt.
69. Speaker and chairperson at 11^th BGICC (nature research course) "Developing of Egyptian networking" 17-18^th Jan. 2019, Cairo, Egypt.
70. Speaker at 8th Pan Arab Hematology Association Congress " Hematogones Detection in Hematological Malignancies and Bone Marrow Transplantation" 1-4^th Feb. 2019, Madinah, Saudi Arabia
71. Trainer in "Approaching plasma cell myeloma diagnostic tools, common versus uncommon" workshop. 20^th March, 2019 at Shefaa Al Orman Hospital, Luxor, Egypt.
72. Speaker at the XII^th International conference of Clinical Pathology Department, Mansoura University. 27^th – 28^th March 2019.
73. EUROMEDLAB, 19-25^th May 2019, Barcelona, Spain.
74. Speaker and chairperson at 12^th BGICC (MASRI conference and workshop) "Traceability of biological materials and associated data" 9-13^th Jan. 2020, Cairo, Egypt
75. Speaker at 38^th ESH conference. 12^th – 13^th Feb. 2020.
76. Speakers at establishing biobank network meeting "Developing biobanks in South Egypt Cancer Institute" 4^th March 2020.
77. Speaker and chairperson at 13^th BGICC (MASRI scientific research course) "Handling and management of research bio-specimens during Covid19 outbreak" 21-22th Jan. 2021, Cairo, Egypt.
78. Speaker at Laboratory Medicine Conference “COVID-19 updates” held on 7^th till 9^th September 2021.
79. 27^th meeting of the Egyptian network of research ethics committees (ENREC), 28^th October, 2021, Cairo, Egypt.
80. Assessment requirements according to ISO/IEC 17011:2017” Accreditation body requirements, 27 November 2021, EGAC, Cairo.
81. 1^st Annual conference of Faculty of Medicine, Suez University, 10-11 March, 2022, Suez, Egypt.
82. Assessor training course ”Accreditation of the biobanks According to the requirements of ISO/IEC 20387:2018” from 24-27 July 2022, EGAC, Cairo.
83. Flow cytometry in hematological malignancies workshop held on October 19, 2022, IMC, Cairo.
84. EPHOS Annual international conference debates & updates in pediatric hematologic malignancies, 6^th – 8^th October, 2022, Cairo, Egypt.
85. Speaker at 12^th conference of clinical pathology department, Tanta Faculty of Medicine, 3,4 November 2022, Tolip Hotel, Alexandria.
86. Speaker in Clinical Pathology workshop validation and quality control of flow cytometry experiments, NCI experience. 10^th November 2022, NCI, Cairo, Egypt.
87. 39^th conference of the ESH, 18^th -19^th January 2023, Cairo, Egypt.
88. Chairperson at 13^th Annual conference of Alexandria Clinical Pathology Department and 16^th Quality Assurance and Laboratory Management Workshop. 8^th – 9^th March 2023, Alexandria, Egypt.
89. Chairperson at XXXII Annual conference of the Egyptian society of laboratory medicine. 6-7^th May 2023, Cairo, Egypt.
90. Participated as a speaker in flowcytometry workshop: future applications of flowcytometry in clinical practice, 6^th November 2023, NCI, Cairo.

1. Constructor of Flow cytometry Lab, SECI (ISO15189 accredited)
2. Constructor of Bone marrow transplant unit in Assiut University (First and only BMT unit in South Egypt)
3. Constructor of Biorepository in SECI, Assiut University
4. Executive director of a project “Development of Flow Cytometry Lab. South Egypt Cancer Institute Assiut University and preparation of Accreditation” From project management at Ministry of Higher Education LP5-011-Assu.
5. Director of the research project “Hepatitis in cancer patients” funded from STDF ID4074.
6. Vice Executive director of a project “Development of Blood Transfusion Center in South Egypt Cancer Institute Assiut University and preparation of Accreditation” From project management at Ministry of Higher Education LP6-21- ASSU.
7. Vice Executive director of a project “Development of Multidisciplinary research center on Excellency in Assiut University”.
8. Director of capacity building project “Development of Flow cytometry laboratory in SECI to be a special research center” funded from STDF ID 4870.
9. Executive director of a project “Development of Hemato-Oncology Lab. South Egypt Cancer Institute Assiut University and preparation of Accreditation” From project management at Ministry of Higher Education LP9-007-Assu

01006261987

1. Member in ISAC (International Society for Analytical Cytology) no 12133.
2. Member in Egyptian Society of Hematology and research.
3. Member in Pan Arab Hematology Association.
4. Treasure of Pan Arab Flowcytometry-Working Group
5. Member in Egyptian Society for progenitor cell research
6. Member in National cancer research network
7. Member in National Biobanking network
8. Member in REGENERA Egypt

Supervisor of MD thesis

1. Apoptosis studies in patients with β thalassemia
2. Flow cytometric platelet cross matching and human leukocyte antigens (HLA) typing for assessment of all immunization in patients with acute leukemia
3. The Use of Flow Cytometer in Measurement of T-Cell reconstitution after treatment of Lymphoproliferative diseases in Pediatric Patients
4. Assessment of dendritic cells and apoptosis in peripheral blood of patients with systemic lupus erythematosis
5. An immunological study on regulatory T cells and complement components in patients with Lupus Nephritis
6. Role of TLR expression in fungal infections in patients with hematological malignancy
7. STAT 3 expression and amplified BCR ABL in chronic myeloid leukemia and their relation to Imatinib therapy
8. Quantitative analysis of complement receptors, CR1 (CD35) and CR3 (CD11b), on neutrophils improves distinction between bacterial and viral infections in chest infection
9.  Biomarkers of endothelial dysfunction and platelet activation in atrial fibrillation
10. Study of minimal residual disease detection as a prognostic factor during treatment of pediatric acute lymphoblastic leukemia at South Egypt Cancer Institute
11.  Detection of Cryptosporidium in Drinking Water in Assiut University Campus and The Effect of Probiotic on its Viability and Infectivity
12.  Giardiasis as a continuing problem and evaluation of anting.
13.  Role of double negative T cell secreting IL17 in systemic lupus erythematosus patients.
14. Targeting protein degradation pathway for therapeutic benefits in multiple myeloma.
15. Role of Factor VIIa – Antithrombin Complex in Pathogenesis and Prediction of Portal Vein Thrombosis in Egyptian Patients with HCV – Related Liver Cirrhosis
16. Quantitation of hematogones at the time of engraftment as a prognostic indicator in allogeneic hematopoietic stem cell transplantation in severe aplastic anemia.
17.  Helicobacter pylori diseases diagnosis and association with immunoregulatory T cells.
18. Role of circulating tumor cells (CTCs) as a prognostic marker in adjuvant setting of early breast cancer patients.
19.  Telomere length as a biological marker in leukemic patients: detection by flow FISH.
20.  Isolation, characterization and biological evaluation of the constituents of Artichoke and its fungal endophytes.
21. Circulation platelet microparticles as a risk of hypercoagulable state in B thalassemia intermedia.
22.  Comprehensive follow up of late effects in childhood cancer survivors at Pediatric Oncology Department South Egypt Cancer Institute.
23.  Re-positioning of the drugs used as second line treatment for adult chronic immune thrombocytopenia ITP.
24.  Expression of CD11a in lymphocyte subpopulation in immune thrombocytopenia.
25.  Derived suppressor cells in patient's effect of tyrosine kinase inhibitors on myeloid with chronic myeloid leukemia.
26. Randomized clinical trial of the use of Eltrombopage in children with idiopathic aplastic anemia.
27.  Cross-talk among regulatory B cells, regulatory T cells and T helper 17 cells and its significance in transplant recipients
28. Evaluation of CD86 marker for minimal residual disease monitoring in B cell precursor acute lymphoblastic leukemia to increase the efficacy of MRD assay.
29. Relationship between immunophenotyping and X-inactive specific transcript [XIST] gene in acute myeloid leukemia.
30. Flow-FISH of CD34+ CD38- for measurement of minimal residual disease in adult acute myeloid leukemia patients.

Supervisor of Master thesis

1. Flow cytometry in hematological diseases
2. The role of flow cytometry in diagnosis of malignant ascites
3. HLA-G molecule and its role in cancer
4. Detection of multidrug resistance in acute leukemia by flowcytometry
5. Autophagy apoptosis relationship in Anthocyanin induced cell death of human T lymphoma cell line “Jurkat”.
6. Red cell microparticles in splenectomized ITP patients compared with nonsplenectomized ITP patients
7. Monocytic tissue factor (CD142) expression in cirrhotic patients with and without type II diabetes mellitus
8. Assessment of bone marrow involvement in Non-Hodgkin’s lymphomas: comparison between immunohistochemistry and flowcytometry.
9. Immunophenotyping of infantile leukemia in South Egypt Cancer Institute
10. The role of peripheral blood CD4⁺CD25^-CD69⁺ T regulatory cells and their expression of membrane bound transforming growth factor β1 in occurrence and severity of hepatocellular carcinoma among cirrhotic infected with hepatitis C virus.
11.  Role of Flowcytometry immunophenotyping in diagnosis and monitoring of multiple myeloma.
12.  Assessment of the use of 8 color flow cytometry in the diagnosis of acute leukemia.
13. Expression of aberrant CD markers in acute leukemia by flowcytometry; retrospective study in South Egypt Cancer Institute.
14. Serum 25-hydroxyvitamin D concentration and Breast Cancer Risk in Egyptian Women in South Egypt.
15. Assessment of humoral immune recovery in children with hematological malignancies after completion of chemotherapy.
16. Differential expression of CD200 in B chronic lymphproliferative disorders by multicolour flow cytometry.

Flow cytometry

        - Hematopathology (very good experience in bone marrow aspiration and biopsy).

        - Immunohematology, blood banking and biobanking

        - Stem cell transplantation and regenerative medicine

https://scholar.google.com/citations?user=R3ZgoakAAAAJ&hl=en

douaa_sayed@hotmail.com