الوصف

After determination of LC 50 , sublethal concentration of water soluble fractions (WSFs) of petroleum crude oil-quarter of LC 50-and same concentrations of zinc and selenium "1.3 mg/l were used to study their effects on reproduction profile of hybrid red tilapia. The experiment was continued for 2 months and the groups were "control, WSFs of petroleum crude oil, zinc, selenium, zinc+ WSFs of petroleum crude oil and selenium+ WSFs of petroleum crude oil". Triplicates of each treatment were established and fish was fed twice/daily on commercial diet 30% protein. By experiment"s end, sex ratio was calculated, gonads were obtained for Gonado-somatic index "GSI", biochemical and histological analysis. Sex ratio was around the normal, 3 females :1 male; results of female GSI showed no significant different between treatments while male GSI showed significant different between treatments compared to control. Steroid"s hormones "estradiol, testosterone and progesterone" showed significant differences between treatments compared to control which has no significant differences with other treatments. The histological analysis showed changes in reproduction organs (testes and ovary) tissues in WSFs of petroleum crude oil treatments, while these changes were eliminated in treatments with WSFs of petroleum crude oil treatment + zinc or selenium; in zinc and selenium treatments the tissue structure closed to be normal compared to the control group.

URL

DOI

https://doi.org/10.21608/asfr.2022.126156.1020

الملخص

الكلمات الدالة

red tilapia,petroleum crude oil, water soluble fractions, chronic effect, Reproduction, gonado-somatic index, histology,gonads